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Detección sistema para la identificación de pequeñas moléculas que promueven la diferenciación celular de células de Glioblastoma de drogas basados en citometría flujo
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1Department of Neurological Surgery, Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine, 2Department of Pathology,Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine
Chapters
- 00:05Title
- 01:17Generation of Lentiviral Transcriptional Reporter Cell Lines
- 03:47Subclone Selection, Expansion, and Validation
- 04:47Determination of GFAP:GFP Expression by Flow Cytometry
- 05:58ELDA Self-renewal Assay to Assess Clonogenic Capacity
- 07:01Drug Screen
- 08:27Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
- 09:46Conclusion
Se presenta un protocolo de investigación eficiente para la identificación de pequeñas moléculas que promueven la diferenciación astroglial en células de glioblastoma (GSCs). El ensayo se basa en un reportero de la diferenciación de células madre por el que la expresión de la GFP mejorada (eGFP) es conducida por el promotor humano de GFAP.
Tags
Flow CytometryDrug ScreeningGlioblastoma Stem CellsCellular DifferentiationLentivirusReporter ConstructHigh-throughput ScreeningSmall MoleculesAstroglial DifferentiationCancer Stem CellsEhlers-Danlos GlioblastomaCell LineageBloodborne PathogensNeural Stem Cell Growth MediumPolybreneDissociation Reagent
