JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
流动 Cytometry-based 药物筛选系统对促进细胞分化胶质母细胞瘤小分子的鉴定
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
流动 Cytometry-based 药物筛选系统对促进细胞分化胶质母细胞瘤小分子的鉴定
DOI:
10.3791/56176-v
•
10:28 min
•
January 10, 2018
•
Raffaella Spina
,
Dillon M. Voss
,
Laura Asnaghi
,
Andrew Sloan
3
,
Eli E. Bar
1
Department of Neurological Surgery, Case Comprehensive Cancer Center
,
Case Western Reserve University School of Medicine
,
2
Department of Pathology
,
Johns Hopkins University, School of Medicine
,
3
Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center
,
Case Western Reserve University School of Medicine
Chapters
00:05
Title
01:17
Generation of Lentiviral Transcriptional Reporter Cell Lines
03:47
Subclone Selection, Expansion, and Validation
04:47
Determination of GFAP:GFP Expression by Flow Cytometry
05:58
ELDA Self-renewal Assay to Assess Clonogenic Capacity
07:01
Drug Screen
08:27
Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
09:46
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
提出了一种有效的筛选方案, 以识别小分子, 促进形分化的胶质母细胞瘤干细胞 (GSCs)。该检测是基于一个干细胞分化的记者, 即增强 GFP (eGFP) 的表达是由人胶质细胞促进剂驱动。
Tags
Flow Cytometry
Drug Screening
Glioblastoma Stem Cells
Cellular Differentiation
Lentivirus
Reporter Construct
High-throughput Screening
Small Molecules
Astroglial Differentiation
Cancer Stem Cells
Ehlers-Danlos Glioblastoma
Cell Lineage
Bloodborne Pathogens
Neural Stem Cell Growth Medium
Polybrene
Dissociation Reagent
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
高通量三维肿瘤球体药物筛选的生成
活体细胞成像技术研究胶质瘤脑干细胞的迁移和侵袭
细胞凋亡逆转后新形成乳腺癌干细胞样细胞的流式细胞仪检测
生理患者衍生的3D类血球体用于抗肿瘤药物筛选,以靶向癌症干细胞
小鼠乳腺皮下HC11和EpH4细胞的分化
利用有机体培养评价小鼠前列腺皮细胞的分化能力
使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器
斑马鱼原发性患者衍生肿瘤异种移植物的药物筛选
胶质母细胞瘤干细胞在模式神经元上共同培养,以研究迁移和细胞相互作用
体内
通过脾T细胞流式细胞术筛选肿瘤来源的细胞外囊泡的免疫原性
Read Article