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受動的な明快さクリア マウス卵巣の血管の 3次元再構築
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Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
Please note that all translations are automatically generated.
Click here for the English version.
受動的な明快さクリア マウス卵巣の血管の 3次元再構築
DOI:
10.3791/56141-v
•
12:38 min
•
December 10, 2017
•
Wei Hu*
1
,
Amin Tamadon*
1
,
Aaron J.W. Hsueh
,
Yi Feng
1
Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine
,
Fudan University
,
2
Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine
,
Stanford University
Chapters
00:00
Title
01:36
Perfusion and Ovary Preparation
03:00
Degassing and Passive CLARITY
05:01
Immunostaining
05:50
Confocal Imaging
08:37
3D Reconstruction of Ovarian Structure
11:04
Results: Representative 3D Passive CLARITY Images of Cleared Ovary
12:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでそのままマウス卵巣における受動的な明快さと卵巣の血管と濾胞性毛細血管の可視化における 3 D 画像再構成法の適応を提案する.
Tags
3D Reconstruction
Vascular Architecture
CLARITY-cleared
Mouse Ovary
Follicle Development
Somatic Cells
Vascular System
Nervous System
Passive CLARITY
Image Analysis
Imaris
Perfusion
Hydrogel
Ovarian Bursa
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