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无源清清除小鼠卵巢血管构筑的三维重建
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Biology
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Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
Please note that all translations are automatically generated.
Click here for the English version.
无源清清除小鼠卵巢血管构筑的三维重建
DOI:
10.3791/56141-v
•
12:38 min
•
December 10, 2017
•
Wei Hu*
1
,
Amin Tamadon*
1
,
Aaron J.W. Hsueh
,
Yi Feng
1
Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine
,
Fudan University
,
2
Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine
,
Stanford University
Chapters
00:00
Title
01:36
Perfusion and Ovary Preparation
03:00
Degassing and Passive CLARITY
05:01
Immunostaining
05:50
Confocal Imaging
08:37
3D Reconstruction of Ovarian Structure
11:04
Results: Representative 3D Passive CLARITY Images of Cleared Ovary
12:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了被动清晰度和3D 重建方法的适应性, 可视化卵巢血管和卵泡毛细血管在完整的小鼠卵巢。
Tags
3D Reconstruction
Vascular Architecture
CLARITY-cleared
Mouse Ovary
Follicle Development
Somatic Cells
Vascular System
Nervous System
Passive CLARITY
Image Analysis
Imaris
Perfusion
Hydrogel
Ovarian Bursa
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