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Biology
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用3D-FRAP显微镜分析miRNA的间隙连接依赖性转移
JoVE Journal
Biology
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JoVE Journal
Biology
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
用3D-FRAP显微镜分析miRNA的间隙连接依赖性转移
DOI:
10.3791/55870-v
•
06:26 min
•
June 19, 2017
•
Heiko Lemcke
2,3
,
Natalia Voronina
2,3
,
Gustav Steinhoff
2,3
,
Robert David
2,3
1
Reference and Translation Center for Cardiac Stem Cell Therapy (RTC)
,
2
Department of Cardiac Surgery
,
University of Rostock
,
3
Department of Life, Light and Matter of the Interdisciplinary Faculty
,
University of Rostock
Chapters
00:05
Title
00:38
Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
01:56
Applying 3D-FRAP Microscopy and Data Analysis
04:08
Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
05:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了光漂白后三维荧光恢复(3D-FRAP)的应用,用于分析miRNA的间隙连接依赖穿梭。与通常应用的方法相比,3D-FRAP允许实时定量小RNA的细胞间转移,具有高的时空分辨率。
Tags
Gap Junction
Micro RNA
3D-FRAP Microscopy
Cardiomyocytes
Cell Culture
Electroporation
Confocal Microscopy
Fluorescence Recovery After Photobleaching (FRAP)
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