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Journal
/
Immunology and Infection
/
表現型および機能解析のためのレア抗原特異的B細胞の検出および濃縮
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Please note that all translations are automatically generated.
Click here for the English version.
表現型および機能解析のためのレア抗原特異的B細胞の検出および濃縮
DOI:
10.3791/55382-v
•
09:25 min
•
February 16, 2017
•
Mia J. Smith
2
,
Thomas A. Packard
,
Shannon K. O’Neill
,
Rochelle M. Hinman
,
Marynette Rihanek
,
Peter A. Gottlieb
,
John C. Cambier
4
1
Department of Immunology and Microbiology
,
University of Colorado School of Medicine
,
2
Department of Microbiology, Immunology, and Pathology
,
Colorado State University
,
3
Barbara Davis Center for Childhood Diabetes
,
University of Colorado School of Medicine
,
4
Department of Biomedical Research
,
National Jewish Health
Chapters
00:05
Title
01:04
Human Peripheral Blood Mononuclear Cell Isolation (PBMC)
02:19
Cell Staining for Magnetic Nanoparticle-based Enrichment
04:41
Magnetic Nanoparticle-based Enrichment
06:51
Results: Representative Tetanus-toxoid-binding B Cell Post-enrichment Analyses
08:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
機能的および表現型分析のために、抗原反応性B細胞を検出し、豊かに磁性ナノ粒子を使用するシンプルで効果的な方法が記載されています。
Tags
Rare Antigen-specific B Cells
Magnetic Nanoparticles
Phenotypic Analysis
Functional Analysis
Immunology
Flow Cytometry
Density Gradient Separation
Mononuclear Cells
Fc Gamma Receptor Blocking
Fluorescence-conjugated Antibodies
Biotinylated Antigen
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