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Journal
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Immunology and Infection
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检测和罕见富集抗原特异性B细胞的表型和功能分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Please note that all translations are automatically generated.
Click here for the English version.
检测和罕见富集抗原特异性B细胞的表型和功能分析
DOI:
10.3791/55382-v
•
09:25 min
•
February 16, 2017
•
Mia J. Smith
2
,
Thomas A. Packard
,
Shannon K. O’Neill
,
Rochelle M. Hinman
,
Marynette Rihanek
,
Peter A. Gottlieb
,
John C. Cambier
4
1
Department of Immunology and Microbiology
,
University of Colorado School of Medicine
,
2
Department of Microbiology, Immunology, and Pathology
,
Colorado State University
,
3
Barbara Davis Center for Childhood Diabetes
,
University of Colorado School of Medicine
,
4
Department of Biomedical Research
,
National Jewish Health
Chapters
00:05
Title
01:04
Human Peripheral Blood Mononuclear Cell Isolation (PBMC)
02:19
Cell Staining for Magnetic Nanoparticle-based Enrichment
04:41
Magnetic Nanoparticle-based Enrichment
06:51
Results: Representative Tetanus-toxoid-binding B Cell Post-enrichment Analyses
08:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
描述了采用磁性纳米颗粒,以检测和富集的功能和表型分析抗原反应性B细胞中的简单而有效的方法。
Tags
Rare Antigen-specific B Cells
Magnetic Nanoparticles
Phenotypic Analysis
Functional Analysis
Immunology
Flow Cytometry
Density Gradient Separation
Mononuclear Cells
Fc Gamma Receptor Blocking
Fluorescence-conjugated Antibodies
Biotinylated Antigen
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