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可追踪记者乳腺癌患者来源的移植瘤贴标肿瘤生长和转移的研究
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Cancer Research
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JoVE Journal
Cancer Research
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
Please note that all translations are automatically generated.
Click here for the English version.
可追踪记者乳腺癌患者来源的移植瘤贴标肿瘤生长和转移的研究
DOI:
10.3791/54944-v
•
09:53 min
•
November 30, 2016
•
Colton Hanna
,
Letty Kwok
,
Jessica Finlay-Schultz
,
Carol A. Sartorius
,
Diana M. Cittelly
1
Department of Pathology, School of Medicine
,
University of Colorado Anschutz Medical Campus
Chapters
00:05
Title
00:58
Dissection and Dissociation of Patient-derived Xenograft (PDX)-derived Tumor Cells
03:20
Human Epithelial Cancer Cell Enrichment
05:13
PDX-derived Tumor Cell Transduction, Transduction Efficiency Evaluation and Tumor Cell Re-implantation
06:48
Results: PDX-dissociated Breast Cancer Cell Transduction Using High Titer Lentiviral Vectors
08:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了源自患者的异种移植物(PDXs)用表达绿色荧光蛋白和荧光素酶记者慢病毒颗粒的稳定标记的方法。该方法允许用于跟踪PDXs的生长在主站点,以及检测
使用体内
成像系统自发和实验转移。
Tags
Breast Cancer
Patient-derived Xenografts
PDX
Reporter Genes
Tumor Growth
Metastasis
In Vivo Imaging
Aseptic Technique
Cell Isolation
Red Blood Cell Lysis
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