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可追踪记者乳腺癌患者来源的移植瘤贴标肿瘤生长和转移的研究
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
DOI:

09:53 min

November 30, 2016

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Chapters

  • 00:05Title
  • 00:58Dissection and Dissociation of Patient-derived Xenograft (PDX)-derived Tumor Cells
  • 03:20Human Epithelial Cancer Cell Enrichment
  • 05:13PDX-derived Tumor Cell Transduction, Transduction Efficiency Evaluation and Tumor Cell Re-implantation
  • 06:48Results: PDX-dissociated Breast Cancer Cell Transduction Using High Titer Lentiviral Vectors
  • 08:33Conclusion

Summary

Automatic Translation

我们描述了源自患者的异种移植物(PDXs)用表达绿色荧光蛋白和荧光素酶记者慢病毒颗粒的稳定标记的方法。该方法允许用于跟踪PDXs的生长在主站点,以及检测使用体内成像系统自发和实验转移。

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