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斑马鱼肾功能评价采用荧光测定间隙
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Biology
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JoVE Journal Biology
Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay
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斑马鱼肾功能评价采用荧光测定间隙

DOI:
10.3791/52540-v

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08:13 min

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February 20, 2015

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, ,
1Genetics and Genomic Medicine, Institute of Child Health,University College London, 2Molecular Cell Science Research Centre,St. George’s University of London

Chapters

  • 00:05Title
  • 01:09Preparation of Instruments and Anesthetic
  • 02:21Zebrafish Husbandry and Pre-injection Treatment
  • 03:33Microinjection
  • 05:27Imaging Acquisition and Processing
  • 07:08Results: Injection of Rhodamine Dextran in bbs9 Morpholino Knockdown Zebrafish
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

斑马鱼是一种流行的工具来模拟慢性肾脏病（CKD）。然而，它们的小尺寸使得它不可能用传统方法评估肾功能。我们描述一个荧光染料肾清除率法1，允许在CKD斑马鱼肾功能定量分析。

Tags

ZebrafishKidneyFluorescent Clearance AssayNephronsPronephric TubulesCiliaKidney FunctionChronic Kidney DiseaseOptical TransparencyRenal Function

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