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1Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie,Université Pierre et Marie Curie, 2Kavli Institute for Brain and Mind,University of California, San Diego, 3Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke,National Institute of Health
Chapters
- 00:05Title
- 01:44Giant Unilamellar Vesicle Growth by Electroformation
- 04:29Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
- 06:07Harvesting and Transferring GUVs
- 07:33Patch-clamping GUVs
- 09:14Results: Microscopy and Patch Clamp Studies of KvAP GUVs
- 11:03Conclusion
A reconstituição da proteína transmembranar, KvAP, em vesículas unilamelares gigantes (GUVs) é demonstrada por dois métodos de desidratação-re-hidratação - eletroformação, e inchaço assistida-gel. Em ambos os métodos, vesículas pequenas unilamelares que contêm a proteína são fundidos em conjunto para formar GUVs que pode, em seguida, ser estudadas por microscopia de fluorescência e de patch-clamp electrofisiologia.
Tags
Transmembrane ProteinVoltage-gated Ion ChannelKvAPGiant Unilamellar Vesicles (GUVs)Biomimetic SystemMembrane Associated PhenomenaDehydration-rehydrationElectroformationGel-assisted SwellingSmall Unilamellar VesiclesRehydration BufferAC FieldAgarose GelFluorescence MicroscopyPatch-clampProtein Distribution
