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核酸,蛋白质与甲基转移和辅酶类似物序列特异性标签
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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Please note that all translations are automatically generated.
Click here for the English version.
核酸,蛋白质与甲基转移和辅酶类似物序列特异性标签
DOI:
10.3791/52014-v
•
12:07 min
•
November 22, 2014
•
Gisela Maria Hanz*
1
,
Britta Jung*
1
,
Anna Giesbertz
,
Matyas Juhasz
,
Elmar Weinhold
1
Institute of Organic Chemistry, Department of Chemistry
,
RWTH Aachen University
Chapters
00:05
Title
02:26
One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
05:07
Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
08:41
Results: Gel Analyses for SMILing and mTAG Reactions
11:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
DNA和蛋白质序列特异性标记的亲和力,或使用DNA或蛋白质的甲基和合成的辅助因子的类似物的荧光报告基团。取决于酶,氮丙啶或双活化的辅助因子类似物的辅因子特异性的用于一个或两个步骤的标签。
Tags
Methyltransferases
S-Adenosyl-l-methionine (AdoMet Or SAM)
DNA Methylation
RNA Methylation
Protein Methylation
Aziridine Cofactors
SMILing DNA
MTAG
Double-activated AdoMet Analogues
Histone Methylation
Click Chemistry
DNA Genotyping
Methylation Detection
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