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丰富的人力细胞系化疗耐药性卵巢癌干细胞
JoVE Journal
Medicine
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JoVE Journal Medicine
Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
DOI:

11:50 min

September 10, 2014

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Chapters

  • 00:05Title
  • 02:02Cell Culture and Fluorescent Labeling Ovarian Cancer Cell Lines
  • 03:39Enrichment for Cancer Stem Cells
  • 04:51CSC Characterization via Gene Expression Analysis for Stem Cell Markers
  • 06:10Analyzing CSCs for Cell Surface Markers CD117 and CD133
  • 07:42Analyzing CSCs for Therapeutic Response
  • 08:41Results: Validation of Ovarian CSCs Enrichment
  • 10:46Conclusion

Summary

Automatic Translation

肿瘤干细胞(肿瘤干细胞)有牵连的肿瘤复发,由于化疗。我们已经进行了优化的协议选择和扩展,从卵巢癌细胞系的CSCs的。通过处理细胞与化疗顺铂和在干细胞培养促进我们丰富的非粘附CSC培养介质。

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