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丰富的人力细胞系化疗耐药性卵巢癌干细胞
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JoVE Journal
Medicine
Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
Please note that all translations are automatically generated.
Click here for the English version.
丰富的人力细胞系化疗耐药性卵巢癌干细胞
DOI:
10.3791/51891-v
•
11:50 min
•
September 10, 2014
•
Jennifer M. Cole
,
Stancy Joseph
,
Christopher G. Sudhahar
,
Karen D. Cowden Dahl
1
Department of Biochemistry and Molecular Biology
,
Indiana University School of Medicine
Chapters
00:05
Title
02:02
Cell Culture and Fluorescent Labeling Ovarian Cancer Cell Lines
03:39
Enrichment for Cancer Stem Cells
04:51
CSC Characterization via Gene Expression Analysis for Stem Cell Markers
06:10
Analyzing CSCs for Cell Surface Markers CD117 and CD133
07:42
Analyzing CSCs for Therapeutic Response
08:41
Results: Validation of Ovarian CSCs Enrichment
10:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肿瘤干细胞(肿瘤干细胞)有牵连的肿瘤复发,由于化疗。我们已经进行了优化的协议选择和扩展,从卵巢癌细胞系的CSCs的。通过处理细胞与化疗顺铂和在干细胞培养促进我们丰富的非粘附CSC培养介质。
Tags
Cancer Stem Cells
Chemoresistance
Ovarian Cancer
SKOV3
OVCA429
Cisplatin
Stem Cell Markers
Oct4
Nanog
Prom1 (CD133)
CD177
CD133
Tumor Relapse
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