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Journal
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Immunology and Infection
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高通量检测到的表型<em>沙门氏菌</em>伤寒协会,侵袭和复制的巨噬细胞
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
High-throughput Assay to Phenotype
Salmonella enterica
Typhimurium Association, Invasion, and Replication in Macrophages
Please note that all translations are automatically generated.
Click here for the English version.
高通量检测到的表型<em>沙门氏菌</em>伤寒协会,侵袭和复制的巨噬细胞
DOI:
10.3791/51759-v
•
11:10 min
•
August 11, 2014
•
Jing Wu
,
Roberta Pugh
,
Richard C. Laughlin
,
Helene Andrews-Polymenis
,
Michael McClelland
,
Andreas J. Bäumler
,
L. Garry Adams
1
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences
,
Texas A&M University
,
2
Department of Microbial and Molecular Pathogenesis, College of Medicine
,
Texas A&M University System Health Science Center
,
3
University of California, Irvine
,
4
Department of Medical Microbiology & Immunology, School of Medicine
,
University of California, Davis
Chapters
00:05
Title
01:59
Murine Macrophage RAW264.7 Cell Culture
03:29
Preparation of
Salmonella
Wild-type and Mutants
05:19
Invasion Assay in a 96-well Culture Plate
09:58
Results: Identification of the Phenotype of
Salmonella
Mutants
10:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高通量测定法
在体外
表现型
沙门氏菌
或其他细菌的关联,侵袭和吞噬细胞与高通量能力的复制被开发。该方法来评估
沙门氏菌
基因敲除突变株的宿主-病原体相互作用的参与。
Tags
Salmonella
Macrophage
Association
Invasion
Replication
High-throughput
Gentamicin Protection Assay
Phenotyping
Deletion Mutants
In Vitro
In Vivo
Article
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