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Rápida identificação de bactérias gram-negativas de Sangue Cultura Caldo Usando MALDI-TOF Espectrometria de Massa
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1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research,Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute,Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney,Westmead Hospital
Chapters
- 00:05Title
- 01:20Preparation of Bacterial Inoculate from Blood Culture Broth
- 05:46Preparation of MALDI-TOF Target Plate
- 06:34MALDI-TOF MS Spectra Acquisition
- 08:42Post Analysis-Interpretation of MALDI-TOF MS Scores
- 10:18Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
- 12:35Conclusion
A aplicação de matriz assistida por laser de dessorção / ionização por tempo de voo (MALDI-TOF), espectrometria de massa (MS) directamente ao caldo de cultura do sangue acelera a identificação de bactérias. O método apresentado é um método rápido e fiável para a identificação de bactérias Gram-negativas directamente do caldo de cultura do sangue.
Tags
Rapid IdentificationGram Negative BacteriaBlood Culture BrothMALDI-TOF Mass SpectrometryClinical MicrobiologyBacterial IdentificationBloodstream InfectionSub-cultureSolid AgarMALDI-TOF MSBlood Culture SpecimensInterfering ResinsProteinsHemoglobinBacterial ConcentrationBacterial PurificationBacterial Extraction
