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Rapida identificazione dei batteri Gram-negativi dal sangue brodo di coltura Uso MALDI-TOF
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1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research,Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute,Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney,Westmead Hospital
Chapters
- 00:05Title
- 01:20Preparation of Bacterial Inoculate from Blood Culture Broth
- 05:46Preparation of MALDI-TOF Target Plate
- 06:34MALDI-TOF MS Spectra Acquisition
- 08:42Post Analysis-Interpretation of MALDI-TOF MS Scores
- 10:18Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
- 12:35Conclusion
L'applicazione di desorbimento laser assistita da matrice tempo / ionizzazione di volo (MALDI-TOF) spettrometria di massa (MS) direttamente emocoltura brodo velocizza l'identificazione dei batteri. Il metodo proposto è un metodo rapido e affidabile per l'identificazione dei batteri Gram negativi direttamente dalla cultura sangue brodo.
Tags
Rapid IdentificationGram Negative BacteriaBlood Culture BrothMALDI-TOF Mass SpectrometryClinical MicrobiologyBacterial IdentificationBloodstream InfectionSub-cultureSolid AgarMALDI-TOF MSBlood Culture SpecimensInterfering ResinsProteinsHemoglobinBacterial ConcentrationBacterial PurificationBacterial Extraction
