Journal
/
/
的IFN-γ酶联免疫斑点检测,以评估水痘 - 带状疱疹病毒特异性细胞免疫继脐血移植的发展
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
DOI:

08:04 min

July 09, 2014

, , , , ,

Chapters

  • 00:05Title
  • 01:27Coating and Blocking the Plates
  • 02:46Cell Plating and Stimulation
  • 04:40Spot Development and Detection
  • 05:54Results: Representative VZV ELISpot Data
  • 07:42Conclusion

Summary

Automatic Translation

新世代的功能测定法如γ干扰素(IFN-γ)的酶联免疫斑点法,其检测细胞因子的产生在单细胞水平,并提供T细胞应答的定量和定性特征,可用于评估细胞介导的​​针对水痘 - 带状疱疹免疫应答病毒(VZV)。

Related Videos

Read Article