Journal
/
/
结合DNA-RNA荧光<em>原位</em>杂交(FISH)研究X染色体失活的分化雌​​性小鼠胚胎干细胞
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
DOI:

15:54 min

June 14, 2014

,

Chapters

  • 00:05Title
  • 02:57Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation
  • 04:55Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis
  • 05:33Labeling of DNA Probes for FISH Experiments
  • 08:02Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH
  • 11:04Post-hybridization Washes and Antibody Mediated Detection of Probe
  • 13:21Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH
  • 14:57Conclusion

Summary

Automatic Translation

荧光原位杂交(FISH)可在细胞内其天然环境中的核酸的检测。我们在这里描述了一种协议,用于组合,同时检测RNA和DNA的鱼的装置,它可以被用来研究X染色体失活在小鼠胚胎干细胞。

Related Videos

Read Article