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抗核抗体筛选使用Hep-2细胞

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Please note that all translations are automatically generated. Click here for the English version.

抗核抗体筛选使用Hep-2细胞

DOI:

10.3791/51211-v

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13:01 min

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June 23, 2014

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Carol Buchner, Cassandra Bryant, Anna Eslami, Gabriella Lakos

¹IFA Development Manager,INOVA Diagnostics, Inc., ²IFA Development,INOVA Diagnostics, Inc., ³Product Manager, Rheumatology,INOVA Diagnostics, Inc., ⁴Medical Director,INOVA Diagnostics, Inc.

Chapters

00:05Title
02:11Sample Preparation and Substrate Selection
03:08Addition of Controls and Samples
04:34Addition of Fluorescent Conjugate
06:08Identification of Positive and Negative Results
07:52Results: Cell Staining Patterns for HEp-2
11:23Conclusion

Summary

Automatic Translation

间接免疫荧光（IIF）的测定法在传统上被用于抗核抗体（ANA）的人血清中的检测。这些抗体的存在可以在全身性自身免疫性风湿病（SARD）帮助诊断。该协议演示了如何有效地进行IIF技术，准确检测这些自身抗体。

Tags

Anti-nuclear Antibody ANA HEp-2 Cells Indirect Immunofluorescence (IIF)Automated Fluorescent Microscopy NOVA View Digital Imaging Systems Slide Processing Fluorescent Microscopy Pattern Recognition Automated Reading Patient Data Integrity Patient Safety

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