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Biology
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从人骨骼肌隔离血管源性多能前体
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
Please note that all translations are automatically generated.
Click here for the English version.
从人骨骼肌隔离血管源性多能前体
DOI:
10.3791/51195-v
•
10:52 min
•
August 21, 2014
•
William C.W. Chen
,
Arman Saparov
3
,
Mirko Corselli
,
Mihaela Crisan
,
Bo Zheng
,
Bruno Péault
8
,
Johnny Huard
1
Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery
,
University of Pittsburgh
,
2
Department of Orthopedic Surgery
,
University of Pittsburgh
,
3
Nazarbayev University Research and Innovation System
,
Nazarbayev University
,
4
Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center
,
University of California at Los Angeles
,
5
Department of Cell Biology
,
Erasmus MC Stem Cell Institute
,
6
OHSU Center for Regenerative Medicine
,
Oregon Health & Science University
,
7
Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine
,
Queen’s Medical Research Institute and University of Edinburgh
,
8
David Geffen School of Medicine and the Orthopaedic Hospital Research Center
,
University of California at Los Angeles
,
9
Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine
,
University of Pittsburgh
Chapters
00:05
Title
01:53
Muscle Biopsy Processing
02:42
Muscle Dissociation and Cell Isolation
05:12
Cell Labelling and Sorting
06:52
Post-sorting Cell Culture
08:08
Results: Human Blood-vessel-derived Stem Cell Subsets
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
人体骨骼肌肉的海港几个多系前驱体的人口,是理想的可再生应用血管内。这种隔离方法允许3多能前体细胞群的同时纯化分别从血管三个结构层次:从内膜肌内皮细胞,从媒体的周细胞,并从外膜外膜细胞。
Tags
Blood-vessel-derived Multipotent Precursors
Mesenchymal Stem/stromal Cells
Myogenic Endothelial Cells
Pericytes
Adventitial Cells
Fluorescence-activated Cell Sorting
Human Skeletal Muscle
Mesodermal Developmental Potentials
Cell Surface Markers
Clinical Applications
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