JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
デュアルカラータイム·ゲーティッド誘導放出枯渇(STED)ナノスによる免疫シナプスの可視化
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Please note that all translations are automatically generated.
Click here for the English version.
デュアルカラータイム·ゲーティッド誘導放出枯渇(STED)ナノスによる免疫シナプスの可視化
DOI:
10.3791/51100-v
•
10:00 min
•
March 24, 2014
•
Emily M. Mace
,
Jordan S. Orange
1
Center for Human Immunobiology
,
Texas Children’s Hospital and Baylor College of Medicine
Chapters
00:05
Title
01:25
Activation of NK Cell on Antibody-coated Coverslips
03:19
Fixing, Permeabilizing, and Staining NK Cells on Coverslips
04:52
Mounting the Coverslips
05:35
STED Imaging
08:21
Optimizing STED Imaging: F-actin in NK Cells
09:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、2色STEDナノスによってガラス上で要約NK細胞の細胞傷害性免疫シナプスを撮像するためのプロトコルを示す。この方法を用いて、我々は、サブ100nmシナプスタンパク質の分解能と細胞骨格を得た。
Tags
Immunological Synapse
Natural Killer Cells
Actin Reorganization
Super-resolution Imaging
Stimulated Emission Depletion (STED) Nanoscopy
Time-gated STED
Cytotoxic Synapse
F-actin Architecture
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
補体の枯渇により特定の細胞集団の枯渇
ビジュアル分析手法と免疫学的データの解析を容易に
マウスのマクロファージの枯渇と再構成
マクロファージコレステロール枯渇との食作用への影響<em>クリプトコックス·ネオフォルマンス</em
グラスサポート平面脂質二重層上のナチュラルキラー細胞免疫シナプスの超解像イメージング
イメージング免疫学的シナプスダイナミクス内皮平面内細胞モデル
IgGおよびIgM自己抗体の同時検出のための二色抗原マイクロアレイの生成
フローサイトメトリーをイメージングを用いた人間システムの免疫シナプスの定性・定量分析
アクチンおよび誘導放出の枯渇 (STED) 顕微鏡を用いた B 細胞免疫シナプスにおける微小管細胞骨格の可視化
免疫シナプス形成中のB細胞におけるオルガネラダイナミクスの研究
Read Article