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免疫突触由双色时间选通受激发射损耗（STED）纳米显微可视化
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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免疫突触由双色时间选通受激发射损耗（STED）纳米显微可视化

DOI:
10.3791/51100-v

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10:00 min

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March 24, 2014

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,
1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:25Activation of NK Cell on Antibody-coated Coverslips
  • 03:19Fixing, Permeabilizing, and Staining NK Cells on Coverslips
  • 04:52Mounting the Coverslips
  • 05:35STED Imaging
  • 08:21Optimizing STED Imaging: F-actin in NK Cells
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们示出了协议，用于成像由两色STED纳米显微NK细胞扼要重述在玻璃上的细胞毒免疫突触。使用这种方法，我们获得的突触蛋白和细胞骨架的亚100纳米分辨率。

Tags

Immunological SynapseNatural Killer CellsActin ReorganizationSuper-resolution ImagingStimulated Emission Depletion (STED) NanoscopyTime-gated STEDCytotoxic SynapseF-actin Architecture

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