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Mess-Wachstum und Genexpression von Tumor-Dynamics Targeted<em> S. Typhimurium</em> Bakterien
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1Health Sciences and Technology,Massachusetts Institute of Technology, 2Department of Bioengineering,University of California, San Diego , 3Biocircuits Institute,University of California, San Diego , 4Molecular Biology Section, Division of Biological Science,University of California, San Diego , 5Broad Institute of Harvard and MIT, 6Department of Medicine,Brigham and Women’s Hospital, 7Electrical Engineering and Computer Science and David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 8Howard Hughes Medical Institute
Chapters
- 00:01Title
- 00:56Cell preparation and subcutaneous injection
- 01:34Subcutaneous injection of cancer cells
- 02:19Bacterial preparation for injection
- 03:01Tail vein injection of bacteria
- 03:44IVIS imaging of bacteria tumors
- 04:11Organ dissection
- 04:43Quantifying bacterial distribution in tumors
- 05:08Discussion and Representative Results
- 07:38Conclusions
Das Ziel dieser Versuche ist es, quantitative zeitliche Verlauf Daten über das Wachstum und die Genexpression Dynamik abgeschwächt erzeugen<em> S. typhimurium</em> Wachsenden Bakterienkolonien in den Tumoren. In diesem Video werden Tumorzelle Vorbereitung und Implantation Bakterien Vorbereitung und Injektion, Ganzkörper-Tier Lumineszenz Imaging, Tumorentfernung und bakterielle Koloniezahlbestimmung.
