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1Department of Oncology,Georgetown University, 2Lombardi Comprehensive Cancer Center,Georgetown University, 3Stem Cell Dynamics,Helmholtz Zentrum München – German Research Center for Environmental Health, 4Department of Medicine,Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science,Dankook University
Chapters
- 00:05Title
- 01:48Generation of Primary Mammary Epithelial Cell Cultures
- 03:39Live-cell Imaging
- 05:56Assessing Cellular Event from Time-lapse Images
- 07:50Making Single Cell Fate Maps with Timm’s Tracking Tool (TTT)
- 09:59Representative Results: Colony Formation in BRCA1 Disrupted Cells
- 11:12Conclusion
Time-lapse imaging viene utilizzato per valutare il comportamento di primarie preneoplastiche cellule epiteliali mammarie derivate da modelli murini geneticamente modificati di rischio di cancro al seno per determinare se ci sono correlazioni tra i parametri specifici di comportamento e distinte lesioni genetiche.
