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Time-lapse imaging of Primary preneoplastische uier epitheelcellen afkomstig van de genetisch gemanipuleerde muis modellen van borstkanker
JoVE Journal
Medicine
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JoVE Journal Medicine
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
DOI:

11:47 min

February 08, 2013

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Chapters

  • 00:05Title
  • 01:48Generation of Primary Mammary Epithelial Cell Cultures
  • 03:39Live-cell Imaging
  • 05:56Assessing Cellular Event from Time-lapse Images
  • 07:50Making Single Cell Fate Maps with Timm’s Tracking Tool (TTT)
  • 09:59Representative Results: Colony Formation in BRCA1 Disrupted Cells
  • 11:12Conclusion

Summary

Automatic Translation

Time-lapse imaging wordt gebruikt om het gedrag van primaire preneoplastische mammaire epitheelcellen afkomstig van genetisch gemanipuleerde muizen met borstkanker te bepalen of er correlaties tussen specifieke gedragsparameters en verschillende genetische laesies beoordelen.

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