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Journal
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Immunology and Infection
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紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
Please note that all translations are automatically generated.
Click here for the English version.
紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞
DOI:
10.3791/50075-v
•
11:31 min
•
January 02, 2013
•
Rafi Chapanian
2
,
Iren Constantinescu
3
,
Donald E. Brooks
2,4
,
Mark D. Scott
2,3
,
Jayachandran Kizhakkedathu
2,4
1
Centre for Blood Research
,
University of British Columbia
,
2
Department of Pathology and Laboratory Medicine
,
University of British Columbia
,
3
Canadian Blood Services
,
University of British Columbia
,
4
Department of Chemistry, Life Sciences Centre
,
University of British Columbia
Chapters
00:05
Title
01:28
Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)
03:30
Whole Blood Collection and Separation of Red Blood Cells (RBC)
04:26
HPG Grafting to RBC
05:28
Characterization of HPG Modified RBC: Complement Mediated Lysis
07:16
Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards
08:08
Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements
09:14
Characterization of HPG Modified RBC: Flow Cytometry Measurements – Protection of Rhesus-D (RhD) Antigens
10:12
Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry
10:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
细胞膜的变形例的红血细胞(红细胞),超支化聚甘油(HPG)。修改红细胞进行了表征双水相分割,渗透脆性和补体介导的溶解。使用流式细胞仪和微的打字系统(MTS)的血液分型卡的表面蛋白抗原的伪装进行了评估。
Tags
Antigen Protection
Red Blood Cells
Membrane Grafting
Hyperbranched Polyglycerol
Alloantibodies
Universal Red Blood Donor
Transfusion Safety
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