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紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
DOI:

11:31 min

January 02, 2013

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Chapters

  • 00:05Title
  • 01:28Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)
  • 03:30Whole Blood Collection and Separation of Red Blood Cells (RBC)
  • 04:26HPG Grafting to RBC
  • 05:28Characterization of HPG Modified RBC: Complement Mediated Lysis
  • 07:16Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards
  • 08:08Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements
  • 09:14Characterization of HPG Modified RBC: Flow Cytometry Measurements – Protection of Rhesus-D (RhD) Antigens
  • 10:12Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry
  • 10:58Conclusion

Summary

Automatic Translation

细胞膜的变形例的红血细胞(红细胞),超支化聚甘油(HPG)。修改红细胞进行了表征双水相分割,渗透脆性和补体介导的溶解。使用流式细胞仪和微的打字系统(MTS)的血液分型卡的表面蛋白抗原的伪装进行了评估。

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