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探讨动脉平滑肌的KV7钾离子通道功能使用膜片钳电生理和压力Myography
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Biology
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JoVE Journal
Biology
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
Please note that all translations are automatically generated.
Click here for the English version.
探讨动脉平滑肌的KV7钾离子通道功能使用膜片钳电生理和压力Myography
DOI:
10.3791/4263-v
•
11:02 min
•
September 14, 2012
•
Lioubov I. Brueggemann
,
Bharath K. Mani
,
Jennifer Haick
,
Kenneth L. Byron
1
Department of Molecular Pharmacology & Therapeutics
,
Loyola University Chicago
Chapters
00:05
Title
02:08
Dissection and Cleaning of Arteries
03:04
Cell Isolation for Patch Clamp Studies
04:50
Whole-cell Recording to Measure Kv7 Potassium Currents or Membrane Voltage
06:36
Use of Intact Artery Segments for Pressure Myography
09:00
Results: Kv7 Potassium Channel Function of Arterial Smooth Muscle using Patch Clamp Electrophysiology and Pressure Myography
10:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
测量KV7(KCNQ)钾离子通道活性的孤立动脉细胞(中与测量大蟒/扩张的反应(使用压力myography),同时采用膜片钳电生理技术)可以揭示有关的重要信息的的KV7渠道作用在血管平滑肌生理和药理学。
Tags
Arterial Smooth Muscle
Kv7 Potassium Channel
Patch Clamp Electrophysiology
Pressure Myography
Smooth Muscle Cells
Resistance Arteries
Artery Diameter
Blood Flow
Systemic Blood Pressure
Cytosolic Calcium Concentration
Ion Transporters
Ion Channels
Signal Transduction Pathways
Vasoactive Hormones
Vasoconstriction
Vasodilation
Therapeutic Agents
Calcium Channel Blockers
Kv7 Voltage-activated Potassium Channels
Physiological Signal Transduction
Rat Mesenteric Arteries
Article
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