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一种实用的新的方法来提取基因组DNA保存血浆蛋白从血液采集套件
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Biology
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
Please note that all translations are automatically generated.
Click here for the English version.
一种实用的新的方法来提取基因组DNA保存血浆蛋白从血液采集套件
DOI:
10.3791/4241-v
•
10:43 min
•
May 18, 2013
•
Jon Waters
,
Vishal Dhere
,
Adam Benjamin
,
Arvind Sekar
,
Archana Kumar
,
Sampath Prahalad
,
David T. Okou
,
Subra Kugathasan
1
Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics
,
Emory University School of Medicine and Children’s Health Care of Atlanta
,
2
Division of Rheumatology, Department of Pediatrics
,
Emory University School of Medicine and Children’s Health Care of Atlanta
Chapters
00:01
Title
01:42
Protocol for DNA Extraction from P100 Tubes
03:13
Lyse Red Blood Cells
03:56
Precipitate Protein
04:13
Prepare Isopropanol Tubes
05:40
Resuspend DNA in DNA Hydration Buffer
06:33
Representative Results
08:20
Comparison to Alternative Methods
09:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们所描述的从全血收集的血浆/血清中分离基因组DNA的新方法。血浆采集后,压实血通常是被丢弃。我们的新方法较现有方法相比,一个显着的改善,使得DNA,不要求额外的血液和血浆可以从一个单一的集合。
Tags
Genomic DNA Extraction
Blood Collection Kits
Plasma Protein Preservation
Laboratory Tests
Blood Collection Tubes
DNA Analysis
Genetic Analysis
Genomic Analysis
Anticoagulated Whole Blood
EDTA-containing Vacutainer
Heparin-containing Tube
Simultaneous Analysis
Parallel Analysis
Proteomic Data
Single Tube Collection Method
Compacted Blood
Sample Processing
Patient Samples
BD P100 Blood Collection System
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