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사후 조직에서 Cardiomyocyte 핵의 분리
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
DOI:

10:12 min

July 10, 2012

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Chapters

  • 00:05Title
  • 02:04Isolation of the Cardiac Nuclei
  • 04:43Immunostaining for Flow Cytometry
  • 05:55Flow Cytometry
  • 07:51Results: Cardiomyocyte Nuclei Isolation by PCM-1 Positivity
  • 09:50Conclusion

Summary

Automatic Translation

심장 핵​​은 밀도 침강 통해 격리 및 유동세포계측법 의해 cardiomyocyte 핵 파악하고 정렬하는 pericentriolar 소재 1 (PCM-1)에 대한 항체로 immunolabeled있다.

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