Journal
/
Medicine
/
验尸报告组织心肌细胞核分离
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
Please note that all translations are automatically generated. Click here for the English version.

验尸报告组织心肌细胞核分离

DOI:
10.3791/4205-v

•

10:12 min

•

July 10, 2012

•

,
1Strategic Research Center for Stem Cell Biology and Cell Therapy,University of Lund, 2Department of Cardiology Lund University Hospital,University of Lund

Chapters

  • 00:05Title
  • 02:04Isolation of the Cardiac Nuclei
  • 04:43Immunostaining for Flow Cytometry
  • 05:55Flow Cytometry
  • 07:51Results: Cardiomyocyte Nuclei Isolation by PCM-1 Positivity
  • 09:50Conclusion

Summary

Automatic Translation

Automatic Translation

心肌细胞核通过密度沉淀分离与1 pericentriolar材料（PCM-1）识别和排序流式细胞仪检测心肌细胞核抗体immunolabeled。

Tags

IsolationCardiomyocyte NucleiDensity SedimentationImmunolabelingAntibodiesPericentriolar Material 1 (PCM-1)Flow Cytometry SortingHigh Throughput AnalysisFrozen Archival MaterialBiobanksCarbon-14 DatingCell-cycle AnalysisThymidine AnaloguesBrdUIdUTranscriptome AnalysisEpigenetic Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从部分Pancreatectomized患者的人类胰岛的分离

癌症干细胞从人类前列腺癌样本隔离

分离和剖析人力胆汁含外来体的MicroRNA-

猪生物医学模型生物库组织样品的取样策略与处理

从成年小鼠分离心膜肌细胞

光遗传学调节心细胞活性的机电评估

使用hiPSC衍生心肌细胞的缺血性心脏病模型和基于视频的心肌细胞收缩比较

从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分，适用于 RNA 分析和巨噬细胞表型分析

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

制备用于心肌梗死后成年小鼠心脏单细胞RNA测序的非心肌细胞悬液

Read Article