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前のDNA精製せずに植物や動物のサンプルの遺伝子型
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Genotyping of Plant and Animal Samples without Prior DNA Purification
Please note that all translations are automatically generated.
Click here for the English version.
前のDNA精製せずに植物や動物のサンプルの遺伝子型
DOI:
10.3791/3844-v
•
11:00 min
•
September 24, 2012
•
Pak Y. Chum
,
Josh D. Haimes
,
Chas P. André
,
Pia K. Kuusisto
,
Melissa L. Kelley
1
Thermo Scientific Molecular Biology Products
,
Thermo Fisher Scientific
Chapters
00:05
Title
01:48
Genotyping of Arabidopsis Plant Individuals with Direct Protocol
04:23
Amplifying Specific DNA Fragments from 15-year-old Oak Leaves using the Dilution Protocol
05:50
Genotyping of Transgenic Mice using the Dilution Protocol
07:05
Results: Direct PCR Analysis
10:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここで紹介するダイレクトPCR法は、未精製の植物および動物組織の少量から直接PCR増幅を容易にします。
Tags
Genotyping
Plant Samples
Animal Samples
DNA Purification
Direct PCR Approach
Thermo Scientific Phusion
Thermo Scientific Phire DNA Polymerases
PCR-based Target DNA Detection
Plant Research
Genotype Analysis
Transgenes
DNA Isolation
Thermo Scientific Phire Plant Direct PCR Kit
Arabidopsis Plant Leaves
Cleaved Amplified Polymorphic Sequence Analysis (dCAPS)
Single Nucleotide Polymorphisms (SNPs)
Restriction Endonuclease Digestion
Phenolic Compounds
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