JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
基因分型的植物和动物样品,恕不另行DNA的纯化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Genotyping of Plant and Animal Samples without Prior DNA Purification
Please note that all translations are automatically generated.
Click here for the English version.
基因分型的植物和动物样品,恕不另行DNA的纯化
DOI:
10.3791/3844-v
•
11:00 min
•
September 24, 2012
•
Pak Y. Chum
,
Josh D. Haimes
,
Chas P. André
,
Pia K. Kuusisto
,
Melissa L. Kelley
1
Thermo Scientific Molecular Biology Products
,
Thermo Fisher Scientific
Chapters
00:05
Title
01:48
Genotyping of Arabidopsis Plant Individuals with Direct Protocol
04:23
Amplifying Specific DNA Fragments from 15-year-old Oak Leaves using the Dilution Protocol
05:50
Genotyping of Transgenic Mice using the Dilution Protocol
07:05
Results: Direct PCR Analysis
10:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这里提出的直接PCR方法便于直接从少量的未精制的植物和动物组织的PCR扩增。
Tags
Genotyping
Plant Samples
Animal Samples
DNA Purification
Direct PCR Approach
Thermo Scientific Phusion
Thermo Scientific Phire DNA Polymerases
PCR-based Target DNA Detection
Plant Research
Genotype Analysis
Transgenes
DNA Isolation
Thermo Scientific Phire Plant Direct PCR Kit
Arabidopsis Plant Leaves
Cleaved Amplified Polymorphic Sequence Analysis (dCAPS)
Single Nucleotide Polymorphisms (SNPs)
Restriction Endonuclease Digestion
Phenolic Compounds
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
无有机溶剂和醋酸,蛋白质与考马斯亮蓝G - 250凝胶染色
从原核文化精简纯化的质粒DNA
一个基于PCR的基因分型方法来区分野生型和观赏品种
白茅</em
从复杂的动物相关样品测序宏基因组和Metatranscriptomes:净化不纯
逆转录病毒整合位点的扩增,新一代测序和基因组DNA制图
高通量CRISPR载体的构建及DNA修饰的表征番茄毛状根的一代
从真核细胞染色体外的环状DNA基因组范围内的净化
混合式切割:用于顽拗植物组织样品的改进切法
扫描电子显微镜(SEM)协议有问题的工厂,卵菌和真菌样本
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
Read Article