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Journal
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Immunology and Infection
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神经元的初步培养体系的研究疱疹病毒潜伏期和活化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
Please note that all translations are automatically generated.
Click here for the English version.
神经元的初步培养体系的研究疱疹病毒潜伏期和活化
DOI:
10.3791/3823-v
•
12:22 min
•
April 02, 2012
•
Mariko Kobayashi
,
Ju-Youn Kim
,
Vladimir Camarena
,
Pamela C. Roehm
,
Moses V. Chao
4,5,6,7
,
Angus C. Wilson
,
Ian Mohr
1
Department of Microbiology
,
New York University School of Medicine
,
2
Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine
,
New York University School of Medicine
,
3
Department of Otolaryngology
,
New York University School of Medicine
,
4
Department of Cell Biology
,
New York University School of Medicine
,
5
Department of Physiology and Neuroscience
,
New York University School of Medicine
,
6
Department of Psychiatry
,
New York University School of Medicine
,
7
Center for Neural Science
,
New York University School of Medicine
Chapters
00:05
Title
01:52
Isolation and Culture of SCG Neurons from Rat Embryos
05:56
Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
08:35
Reactivation and Assessment
09:45
Results: HSV-1 Reactivation
11:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
协议描述了一种高效和可重复性的模型系统研究单纯疱疹病毒1型(HSV-1)延迟和重新启动。该法采用同质交感神经神经元文化,并允许使用各种工具,包括RNA干扰和重组蛋白表达的病毒神经元相互作用的分子解剖。
Tags
Primary Neuron Culture System
Herpes Simplex Virus
Latency
Reactivation
Antivirals
Vaccines
In Vivo Studies
Small Animal Models
Viral Gene Requirements
Immune Responses
Neuron-only System
Sympathetic Neurons
Rat Superior Cervical Ganglia (SCG)
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