/

/

抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Please note that all translations are automatically generated. Click here for the English version.

抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像

DOI:

•

09:49 min

•

March 07, 2012

•

Andy Y. Shih, Celine Mateo, Patrick J. Drew³, Philbert S. Tsai, David Kleinfeld⁴

¹Department of Physics,University of California, San Diego, ²Department of Engineering Science and Mechanics,Pennsylvania State University , ³Department of Neurosurgery,Pennsylvania State University , ⁴Section of Neurobiology,University of California, San Diego

Chapters

00:05Title
01:40Surgery Preparation and Animal Mounting
04:02The Generation of a Polished and Reinforced Thinned-skull Window
07:14Imaging Preparation
08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
09:30Conclusion

Summary

Automatic Translation

我们提出了一个方法，形成一个横跨毫米，是没有大脑的炎症个月稳定在小鼠颅骨的影像窗口。这种方法非常适合于使用双光子显微镜的血液流通，细胞动力学，细胞/血管结构的纵向研究。

Tags

Polished And Reinforced Thinned-skull Window Long-term Imaging Mouse Brain In Vivo Imaging Cortical Function Optical Access Intracranial Environment PoRTS Window Optical Clarity Bone Regrowth Inhibition Light Scattering Reduction Inflammation Reduction Two-photon Laser Scanning Microscopy Cerebral Blood Flow Cellular Function Anesthetized Preparations Awake Preparations Optogenetics Blood Flow Disruption

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

慢性成像小鼠的视觉皮层使用减薄，颅骨的制备

活体鼠脑微循环显微镜使用一个封闭的颅窗口

多鼠神经解剖学磁共振成像

灭绝培训在彭兰霞窗口无法还原的恐惧

长期的嗅觉适应性的分子读数<em> C.线虫</em

稀疏的头骨皮层的窗口技术<em在体内</em光学相干断层成像

长时程增强一个非常稳定的和可重复的记录和保存海马鼠标片制备工艺的改进

双光子<em>在体内</em>树突棘中的鼠标皮质用稀疏的头颅成像准备

老鼠耳蜗外植体的长期时间推移成像

琼脂糖微室的长期钙成像<em>秀丽隐杆线虫</em

Read Article