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Immunology and Infection
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特定の遺伝子の過剰発現やノックダウン後にラモスB細胞に体細胞高頻度変異の評価
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
Please note that all translations are automatically generated.
Click here for the English version.
特定の遺伝子の過剰発現やノックダウン後にラモスB細胞に体細胞高頻度変異の評価
DOI:
10.3791/3573-v
•
08:12 min
•
November 01, 2011
•
Dana C. Upton
,
Shyam Unniraman
1
Department of Immunology
,
Duke University
Chapters
00:05
Title
01:00
Transfecting BOSC 23 Cells and Harvesting Viral Particles
02:27
Infecting B Cells
03:34
Single Cell Seeding
04:42
Analysis: IgM Loss (3 Weeks and 5 Weeks)
05:57
Representative Effects of Overexpressing Activated-induced Cytidine Deaminase (AID) or Knocking Down a Repair Factor in Ramos Cells
07:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、過剰発現またはshRNAを含む人間ラモスB細胞株とどのようにこれらの細胞で体細胞高頻度変異を測定するためのコンストラクトのレトロウイルスやレンチウイルス感染症を実行する方法について説明します。
Tags
Somatic Hypermutation
Ramos B Cells
Overexpression
Knockdown
Specific Genes
V(D)J Recombination
Affinity Antibodies
Immunoglobulin Gene
Somatic Hypermutation (SHM)
Class Switch Recombination (CSR)
Activation-induced Cytidine Deaminase (AID)
Cytosine Residues
Uracils
Repair Pathways
Molecular Details
Mice Models
Genetic Knockouts
Repair Factors
Double-strand Break Repair Proteins
B-cell Development
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