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Immunology and Infection
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评估拉莫斯B细胞的特定基因的过表达或击倒后的体细胞超突变
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Immunology and Infection
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Immunology and Infection
Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
Please note that all translations are automatically generated.
Click here for the English version.
评估拉莫斯B细胞的特定基因的过表达或击倒后的体细胞超突变
DOI:
10.3791/3573-v
•
08:12 min
•
November 01, 2011
•
Dana C. Upton
,
Shyam Unniraman
1
Department of Immunology
,
Duke University
Chapters
00:05
Title
01:00
Transfecting BOSC 23 Cells and Harvesting Viral Particles
02:27
Infecting B Cells
03:34
Single Cell Seeding
04:42
Analysis: IgM Loss (3 Weeks and 5 Weeks)
05:57
Representative Effects of Overexpressing Activated-induced Cytidine Deaminase (AID) or Knocking Down a Repair Factor in Ramos Cells
07:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了如何执行逆转录病毒或慢病毒感染的过度表达或shRNA包含人类拉莫斯的B -细胞系构造和如何衡量在这些细胞中的体细胞超突变。
Tags
Somatic Hypermutation
Ramos B Cells
Overexpression
Knockdown
Specific Genes
V(D)J Recombination
Affinity Antibodies
Immunoglobulin Gene
Somatic Hypermutation (SHM)
Class Switch Recombination (CSR)
Activation-induced Cytidine Deaminase (AID)
Cytosine Residues
Uracils
Repair Pathways
Molecular Details
Mice Models
Genetic Knockouts
Repair Factors
Double-strand Break Repair Proteins
B-cell Development
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