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定量比较
顺
调节元件(CRE)的活动,在转基因
果蝇</em
JoVE Journal
Biology
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Biology
Quantitative Comparison of
cis
-Regulatory Element (CRE) Activities in Transgenic
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
定量比较
顺
调节元件(CRE)的活动,在转基因
果蝇</em
DOI:
10.3791/3395-v
•
08:19 min
•
December 19, 2011
•
William A. Rogers
,
Thomas M. Williams
1
Department of Biology
,
University of Dayton
,
2
Department of Biology, Center for Tissue Regeneration and Engineering at Dayton
,
University of Dayton
Chapters
00:05
Title
01:34
Obtaining and Staging Metamorphic Flies
02:49
Steps to Remove Specimen from Puparium
03:33
Confocal Microscopic Evaluation of Reporter Gene Expression in a Whole Mount Sample
04:49
Quantifying cis-Regulatory Element Activity with ImageJ
05:34
Utility of Quantitative CRE Activity
07:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
性状的表型变异可能导致的顺式调控元件(CRE)序列控制的基因表达模式的突变。在果蝇中使用派生的方法可以定量比较的空间和时间修改或自然发生的综合招聘考试的变体介导的基因表达模式的水平。
Tags
Cis-regulatory Element
CRE Activities
Gene Expression Patterns
Enhancers
Cis-regulatory Modules
Transcription Factor Proteins
Regulatory Logic
Development Program
Morphological Evolution
Genetic Variation
Phenotypic Variation
Reporter Transgenes
In Vivo Function
Heterologous Promoter
Coding Sequences
Reporter Gene
Protein Product
P-element Mediated Transgenesis
Drosophila Melanogaster
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