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Chapters
- 00:05Title
- 01:36Staining Nematodes with DiI
- 04:24Mounting DiI-stained Nematodes
- 05:40DiI Staining of C. elegans Cuticle
- 07:52Conclusion
Biz canlı manikür görselleştirmek için bir yöntem mevcut<em> C. elegans</em> Yaygın olarak kullanılan kırmızı floresan lipofilik boya DII (1,1 '-dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine perklorat)<em> C. elegans</em> Çevreye maruz kalan nöronlar görselleştirmek. Alae ve halkalı cuticular yapılar optimize edilmiş bu protokol ile, DII tarafından boyanmış ve bileşik mikroskobu kullanarak gözlenen.
Tags
VisualizationCaenorhabditis ElegansCuticular StructuresLipophilic Vital Dye DiICuticleResistant StructureEnvironment ProtectionBody ShapeMotilityEpidermal CellsLipid LayerCircumferential RidgesAnnuliAlaeDevelopment StagesMutationsCollagen OrganizationCompound MicroscopyDIC OpticsFluorescent Transgene ExpressionAntibody StainingElectron MicroscopyWheat Germ Agglutinin (WGA)Cuticular GlycoproteinsFluorescent DyeLive Imaging
