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可视化线虫角质层结构的亲脂性的重要染料，直接投资收益
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JoVE Journal Biology
Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI
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可视化线虫角质层结构的亲脂性的重要染料，直接投资收益

DOI:
10.3791/3362-v

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08:22 min

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January 30, 2012

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,
1Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center, College of Medicine

Chapters

  • 00:05Title
  • 01:36Staining Nematodes with DiI
  • 04:24Mounting DiI-stained Nematodes
  • 05:40DiI Staining of C. elegans Cuticle
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一个可视化生活角质层的方法<em> C。线虫</em>使用红色荧光亲脂性染料DII（1,1' - 双十八烷基- 3，3,3'，3' - tetramethylindocarbocyanine高氯酸盐），这是常用的<em> C。线虫</em>可视化环境接触的神经元。这种优化的协议，alae和环形表皮结构是由DII染色和使用复合显微镜观察。

Tags

VisualizationCaenorhabditis ElegansCuticular StructuresLipophilic Vital Dye DiICuticleResistant StructureEnvironment ProtectionBody ShapeMotilityEpidermal CellsLipid LayerCircumferential RidgesAnnuliAlaeDevelopment StagesMutationsCollagen OrganizationCompound MicroscopyDIC OpticsFluorescent Transgene ExpressionAntibody StainingElectron MicroscopyWheat Germ Agglutinin (WGA)Cuticular GlycoproteinsFluorescent DyeLive Imaging

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