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Journal
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Immunology and Infection
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在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Please note that all translations are automatically generated.
Click here for the English version.
在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌
DOI:
10.3791/3344-v
•
11:09 min
•
October 23, 2011
•
Tim J. Dumonceaux
,
Jennifer R. Town
,
Janet E. Hill
,
Bonnie L. Chaban
,
Sean M. Hemmingsen
1
Saskatoon Research Centre
,
Agriculture and Agri-Food Canada
,
2
Department of Veterinary Microbiology
,
University of Saskatchewan
,
3
Plant Biotechnology Institute
,
National Research Council of Canada
Chapters
00:05
Title
03:05
Coupling Oligonucleotide Probes to Fluorescent Beads
06:37
Production of cpn60 Universal Target Amplicon and Generation of Single Strands
09:36
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个复用的微生物样本内使用寡核苷酸耦合的荧光珠的检测方法。从样本内的所有生物体的扩增杂交的探针耦合珠小组。一个Luminex公司或生物复杂的仪器是用于查询每个珠珠型和杂交信号。
Tags
Multiplex Detection
Bacteria
Clinical Samples
Environmental Samples
Oligonucleotide-coupled Fluorescent Microspheres
Bacterial Vaginosis
Polymicrobial Syndrome
Lactobacillus
Gardnerella Vaginalis
Atopobium Vaginae
Negative Health Outcomes
HIV Acquisition
Clinical Management
Diagnosis
Gram Stains
Vaginal Swab Smears
Subjective Interpretations
Vaginal Microbiota Composition
Deep Sequencing
Molecular Diagnosis
Article
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