Journal
/
/
追踪中性粒细胞腔内的爬行,活体视频显微镜Transendothelial组织中的迁移和趋化
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
DOI:

15:01 min

September 24, 2011

, ,

Chapters

  • 00:05Title
  • 01:50Introduction
  • 02:42Preparation of Chemoattractant in Agarose Gel
  • 04:30Preparation of Cremaster Muscle for Intravital Microscopy
  • 10:14Cell Tracking Using ImageJ
  • 12:18Analysis of Neutrophil Recruitment Parameters

Summary

Automatic Translation

我们描述了协议的明源在体内的中性粒细胞趋化中性粒细胞招聘时测量动态的中性粒细胞 - 内皮细胞相互作用的活体显微镜。中性粒细胞腔内爬行,transendothelial迁移和趋化鼠标提睾肌组织与时间已过视频摄影和ImageJ跟踪观察。

Related Videos

Read Article