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Chapters
- 00:05Title
- 01:34Day 1: Viral Infection and Preparation of Buffers
- 02:56Day 2, Phase 1: Cell Lysis and Ultracentrifugation
- 04:01Day 2, Phase 2: Ultracentrifugation
- 07:35“Ghost” DNA Precipitation
- 09:56Representative Results
- 11:51Conclusion
Descrevemos o processo de isolamento de DNA de alta pureza herpesvirus nucleocapsídeo das células infectadas. O DNA final capturada a partir de solução é de alta concentração e pureza, tornando-a ideal para high-throughput seqüenciamento de alta fidelidade reações PCR e transfections para produzir novos recombinantes viral.
Tags
Viral DNANucleocapsidsHost Cell ContaminantsDownstream ApplicationsViral Genome SequencingHost DNA RemovalViral Recombinant StrainsPurified PlasmidLinear Viral DNAExtractionsDensity-based CentrifugationPurified Viral CapsidsCellular ProteinsOD260/280 RatioYields Of Viral DNALong-term StorageHigh-throughput SequencingHigh Fidelity PCR ReactionsTransfections
