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Journal
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Immunology and Infection
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从核衣壳的病毒DNA的制备
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Preparation of Viral DNA from Nucleocapsids
Please note that all translations are automatically generated.
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从核衣壳的病毒DNA的制备
DOI:
10.3791/3151-v
•
12:45 min
•
August 16, 2011
•
Moriah L. Szpara
,
Yolanda R. Tafuri
,
L. W. Enquist
1
Department of Molecular Biology
,
Princeton University
Chapters
00:05
Title
01:34
Day 1: Viral Infection and Preparation of Buffers
02:56
Day 2, Phase 1: Cell Lysis and Ultracentrifugation
04:01
Day 2, Phase 2: Ultracentrifugation
07:35
“Ghost” DNA Precipitation
09:56
Representative Results
11:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了从被感染的细胞中分离高纯度的疱疹病毒衣壳DNA的过程。从溶液中捕获的是最后的DNA浓度和纯度高,使得它非常适合高通量测序,高保真PCR反应,并产生新的病毒重组体转染。
Tags
Viral DNA
Nucleocapsids
Host Cell Contaminants
Downstream Applications
Viral Genome Sequencing
Host DNA Removal
Viral Recombinant Strains
Purified Plasmid
Linear Viral DNA
Extractions
Density-based Centrifugation
Purified Viral Capsids
Cellular Proteins
OD260/280 Ratio
Yields Of Viral DNA
Long-term Storage
High-throughput Sequencing
High Fidelity PCR Reactions
Transfections
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