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Biology
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Barcoded 효모 게놈 라이브러리의 경쟁력 화면
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Competitive Genomic Screens of Barcoded Yeast Libraries
Please note that all translations are automatically generated.
Click here for the English version.
Barcoded 효모 게놈 라이브러리의 경쟁력 화면
DOI:
10.3791/2864-v
•
11:59 min
•
August 11, 2011
•
Andrew M. Smith*
1,2
,
Tanja Durbic*
2,3
,
Julia Oh*
4
,
Malene Urbanus
2
,
Michael Proctor
,
Lawrence E. Heisler
3
,
Guri Giaever
6
,
Corey Nislow
2,3
1
Banting and Best Department of Medical Research and Department of Molecular Genetics
,
University of Toronto
,
2
Donnelly Centre for Cellular and Biomolecular Research
,
University of Toronto
,
3
Donnelly Sequencing Centre
,
University of Toronto
,
4
Genetics and Molecular Biology Branch
,
National Human Genome Research Institute, NIH
,
5
Stanford Genome Technology Center, Stanford School of Medicine
,
Stanford University
,
6
Department of Pharmaceutical Sciences
,
University of Toronto
Chapters
00:05
Title
01:55
Combining Individual Mutants into a Single Pool
03:23
Experimental Pool Growth
04:56
Genomic DNA Extraction, PCR and Microarray Hybridization
08:33
Sequencing Barcoded Yeast Strains
09:22
Genomic Screening Results
11:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
우리는 유전자 - 약물 및 유전자 - 환경 상호 작용을 이해하는 포괄적인 불편 게놈 - 와이드 스크린을 개발했습니다. 이러한 돌연변이 컬렉션을 검사 방법이 제공됩니다.
Tags
Competitive Genomic Screens
Barcoded Yeast Libraries
Next Generation Sequencing
Genome Sequences
Gene Function
Deletion Mutants
High-throughput Phenotyping
DNA Tags
Barcodes
Mutant Fitness
Transposon Disruption-barcoding Approach
Candida Albicans Barcoded Disruption
Article
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