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バーコード酵母ライブラリーの競争力のあるゲノム画面
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Competitive Genomic Screens of Barcoded Yeast Libraries
Please note that all translations are automatically generated.
Click here for the English version.
バーコード酵母ライブラリーの競争力のあるゲノム画面
DOI:
10.3791/2864-v
•
11:59 min
•
August 11, 2011
•
Andrew M. Smith*
1,2
,
Tanja Durbic*
2,3
,
Julia Oh*
4
,
Malene Urbanus
2
,
Michael Proctor
,
Lawrence E. Heisler
3
,
Guri Giaever
6
,
Corey Nislow
2,3
1
Banting and Best Department of Medical Research and Department of Molecular Genetics
,
University of Toronto
,
2
Donnelly Centre for Cellular and Biomolecular Research
,
University of Toronto
,
3
Donnelly Sequencing Centre
,
University of Toronto
,
4
Genetics and Molecular Biology Branch
,
National Human Genome Research Institute, NIH
,
5
Stanford Genome Technology Center, Stanford School of Medicine
,
Stanford University
,
6
Department of Pharmaceutical Sciences
,
University of Toronto
Chapters
00:05
Title
01:55
Combining Individual Mutants into a Single Pool
03:23
Experimental Pool Growth
04:56
Genomic DNA Extraction, PCR and Microarray Hybridization
08:33
Sequencing Barcoded Yeast Strains
09:22
Genomic Screening Results
11:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は包括的、公平なゲノムワイドな遺伝子薬剤と遺伝子 - 環境相互作用を理解するために画面を開発しました。これらの変異体のコレクションをスクリーニングするための方法が提示されます。
Tags
Competitive Genomic Screens
Barcoded Yeast Libraries
Next Generation Sequencing
Genome Sequences
Gene Function
Deletion Mutants
High-throughput Phenotyping
DNA Tags
Barcodes
Mutant Fitness
Transposon Disruption-barcoding Approach
Candida Albicans Barcoded Disruption
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