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Lazer Uncagable Floresein Dextran'ın Zebra balığı Hücre Lineage Etiketleme
JoVE Journal
Biology
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JoVE Journal Biology
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
DOI:

07:35 min

April 28, 2011

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Chapters

  • 00:05Title
  • 01:05Injection of Caged Fluorescein Dextran into Zebrafish Embryos
  • 02:49Laser Uncaging of Fluorescein Dextran
  • 04:14Antibody Labeling and Detection of Uncaged Fluorescein Dextran
  • 06:36Representative Immunolabeling Results
  • 07:19Conclusion

Summary

Automatic Translation

Bu protokol, Uncaged floresein gelen sinyali yükseltmek için immunolabeling tüm montaj takip kafesli flöresein UV uncaging kullanarak hücrelerin zebrafish embriyoların küçük gruplar kaderi etiket ve takip etmek için bir yol çizer.

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