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天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖
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Biology
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JoVE Journal
Biology
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
Please note that all translations are automatically generated.
Click here for the English version.
天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖
DOI:
10.3791/2672-v
•
07:35 min
•
April 28, 2011
•
Joshua A. Clanton
,
Ilya A. Shestopalov
,
James K. Chen
,
Joshua T. Gamse
1
Department of Biological Sciences
,
Vanderbilt University
,
2
Department of Chemical and Systems Biology
,
Stanford University
Chapters
00:05
Title
01:05
Injection of Caged Fluorescein Dextran into Zebrafish Embryos
02:49
Laser Uncaging of Fluorescein Dextran
04:14
Antibody Labeling and Detection of Uncaged Fluorescein Dextran
06:36
Representative Immunolabeling Results
07:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描绘的方式,标签和跟踪的小斑马鱼的胚胎细胞群体,使用紫外线uncaging笼荧光,整个装载免疫标记uncaged荧光信号放大的命运。
Tags
Lineage Labeling
Zebrafish Cells
Laser Uncagable Fluorescein Dextran
Developmental Biology
Cell Types
Lineage Labeling Methods
DiI Labeling
Traceable Enzymes
Zebrafish Labs
Photoconvertable Fluorescent Proteins
Eos
Kaede
Ultraviolet Light
Transgenesis
Dye-mediated Lineage Labeling Methods
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