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Chapters
- 00:05Title
- 01:27Phosphorus Extraction and pH Adjustment
- 02:13Enzyme Stock Preparation
- 02:59P Calibration Curve and Controls
- 04:23Sample + Enzyme Incubation
- 05:20Colorimetric Measurement of Released and Background Inorganic P
- 06:09Classification of P Compounds
- 07:35HTP Analysis of Phosphorus in Soil
- 08:26Conclusion
Dieses Protokoll beschreibt eine High-Throughput-Verfahren zur enzymatischen Hydrolyse, dass ein Mikroplatten-Reader nutzt, um zu messen und zu klassifizieren Boden Phosphor als P Mono-, Di-P und anorganischen P. Bis zu 96 Proben auf einmal in einem Standard-Labor gemessen werden.
Tags
High-throughput MeasurementClassificationOrganic PhosphorusEnvironmental SamplesOrganic P CompoundsTraditional P MeasurementsColorimetric ReagentsEnzymatic HydrolysisAccurate CharacterizationP FormsPhosphorus-31 Nuclear Magnetic Resonance Spectroscopy31P-NMRExpensive MethodSpecialized Technical TrainingAdapted Enzymatic Hydrolysis MethodMicroplate Reader SystemFast And Accurate MeasurementAffordable MethodUser-friendly MeansP Species In SoilsSedimentsManuresAquatic SamplesHigh-throughput MethodForms And Enzyme-lability Of Organic PStandard LaboratorySystem Nutrient ContentEutrophication Potential
