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高通量测量和环境样品中的有机磷分类
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
High-Throughput Measurement and Classification of Organic P in Environmental Samples
Please note that all translations are automatically generated.
Click here for the English version.
高通量测量和环境样品中的有机磷分类
DOI:
10.3791/2660-v
•
08:58 min
•
June 08, 2011
•
Nicholas R. Johnson
,
Jane E. Hill
1
School of Engineering
,
University of Vermont
Chapters
00:05
Title
01:27
Phosphorus Extraction and pH Adjustment
02:13
Enzyme Stock Preparation
02:59
P Calibration Curve and Controls
04:23
Sample + Enzyme Incubation
05:20
Colorimetric Measurement of Released and Background Inorganic P
06:09
Classification of P Compounds
07:35
HTP Analysis of Phosphorus in Soil
08:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此协议,介绍了一种酶水解,利用酶标仪来衡量和分类,土壤磷P单酯,磷酯和无机体育多达96个样品,可以在一次在一个标准的实验室测量高通量的方法。
Tags
High-throughput Measurement
Classification
Organic Phosphorus
Environmental Samples
Organic P Compounds
Traditional P Measurements
Colorimetric Reagents
Enzymatic Hydrolysis
Accurate Characterization
P Forms
Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy
31P-NMR
Expensive Method
Specialized Technical Training
Adapted Enzymatic Hydrolysis Method
Microplate Reader System
Fast And Accurate Measurement
Affordable Method
User-friendly Means
P Species In Soils
Sediments
Manures
Aquatic Samples
High-throughput Method
Forms And Enzyme-lability Of Organic P
Standard Laboratory
System Nutrient Content
Eutrophication Potential
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