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Neuroscience
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测定在异种细胞表面的化学感应受体的表达
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Neuroscience
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
Please note that all translations are automatically generated.
Click here for the English version.
测定在异种细胞表面的化学感应受体的表达
DOI:
10.3791/2405-v
•
04:55 min
•
February 23, 2011
•
Sandeepa Dey
,
Senmiao Zhan
,
Hiroaki Matsunami
1
Department of Molecular Genetics and Molecular Biology
,
Duke University
,
2
Department of Chemistry
,
Duke University
Chapters
00:05
Title
01:25
Transfection of HEK293T Cells and Plating for Surface Expression Assay
02:47
Cell Surface Staining of Receptors
04:12
Cell Surface Staining of the Odorant Receptor Olfr62
04:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们展示了一个协议,进行活细胞染色,可用于检测HEK293T细胞表面上的气味受体方便。此外,它也可能被用来检测其他化学感受受体或GPCRs的表面表达。
Tags
Assaying
Surface Expression
Chemosensory Receptors
Heterologous Cells
Olfaction
G Protein Coupled Receptors (GPCRs)
Odorant Molecules
Intracellular Signal
G Protein Gαolf
Combinatorial Rule
Biochemical Studies
Ligand Binding Studies
Heterologous Cells
Receptor Transporting Protein (RTP)
Olfactory Sensory Neurons
Chaperones
Transfection
Biochemical Assays
Cell Line
Live Cell Staining
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