JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
集落形成细胞(CFC)的含量对人体的造血细胞
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
Please note that all translations are automatically generated.
Click here for the English version.
集落形成细胞(CFC)的含量对人体的造血细胞
DOI:
10.3791/2195-v
•
11:30 min
•
December 18, 2010
•
Nayan J. Sarma
,
Akiko Takeda
,
Nabeel R. Yaseen
1
Department of Pathology and Immunology
,
Washington University School of Medicine
Chapters
00:05
Title
01:04
Introduction
01:17
Thawing and Pre-Activation of CD34+ Cells
03:25
Retroviral Transduction by Virus Pre-Loading
05:04
Cell Sorting and Collection of Transduced Cells
06:08
CFC Assay
08:14
Giemsa Staining
10:29
CFC Assay Results with Geimsa Stain Analyses
11:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
集落形成细胞(CFC)的检测是在体外实验中,造血祖细胞形成半固体培养基中的菌落。结合菌落形态,细胞形态,流式细胞仪是用来评估的祖细胞的增殖和分化沿不同的造血谱系能力。
Tags
Colony Forming Cell (CFC) Assay
Human Hematopoietic Cells
Bone Marrow
Cord Blood
Peripheral Blood
Hematopoiesis
Leukemogenesis
Lymphoid Lineage
Myeloid Lineage
Semisolid Medium
Proliferation
Differentiation Pattern
Colonies
Input Cells
Flow Cytometry
Giemsa-stained Slides
Myeloid Differentiation
Oncogenes
Primary Human CD34+ Cells
Retroviral Construct
NUP98-HOXA9
MSCV-IRES-GFP
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Multivirus特异性T细胞的生成,预防/治疗异基因造血干细胞移植后的病毒感染
HLA - IG基于人工抗原提呈细胞可高效
体外
扩展人类的CTL
在细胞命运<em> E.大肠杆菌</em感染后的噬菌体λ
使用γ-干扰素酶酶联免疫检测的特点人类乳头状瘤病毒的新型T-细胞表位
piggyBac转座子
初级人类T细胞系统改造
定量高通量单细胞的细胞毒性T细胞的测定
在纯化造血干/祖细胞识别DNA突变
杀手人工抗原提呈细胞(KaAPC)为有效<em>在体外</em>人抗原特异性T细胞耗竭
巨噬细胞集落刺激因子分化的人单核细胞衍生的巨噬细胞的培养
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
Read Article